CloneID: 4-4-20 (enhanced)
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with fluorescein I-keyhole limpet hemocyanin (FlI-KLH).
Origin Pub PMID: 10556036
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric goat antibody was made using the variable domain sequences of the original mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for fluorescein I.
Application Notes (Clone): This antibody, a humanized, enhanced scFv of the original mouse IgG2a antibody, was constructed by grafting the CDRs of the original antibody onto the humanized 4D5 anti-HER2 antibody framework, and subsequently enhancing it with respect to folding and stability via randomization in the form of DNA shuffling, as well as oligonucleotides encoding mutations at defined positions (Jung et al., 1999; PMID: 10556036). The original antibody was tested against FlI-BSA. The enhanced antibody fragment was tested against FITC-BSA. This antibody can be used as an isotype control in both in vitro and in vivo applications. This antibody is not conjugated to fluorescein.
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