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Applications: Useful for labeling cells expressing GITR for flow cytometry and immunofluorescence microscopy.
Assay Conditions: For flow cytometry, we recommend incubating cells with 20 µg/ml of anti-human PD-1 antibody, PE-labeled for one hour at 4°C, followed by two washes with FACS buffer.
Description: Phycoerythrin-labeled anti-GITR recombinant human antibody recognizing the GITRL binding region of human GITR. This antibody has not been tested for cross-reactivity with any other species.
Format: Aqueous buffer solution
Formulation: 8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol
Purification: Protein A affinity chromato-graphy from HEK293 supernatants.
Storage Stability: Stable for at least 12 months at -80°C. Protect from light. Avoid freeze/thaw cycles. Store diluted antibody with carrier protein (BSA 0.1 –0.5%) at 4°C, stable for 2 weeks.
Uniprot: Q9Y5U5
Warnings: Avoid freeze/thaw cycles. Protect from light.
Biosafety Level: Not applicable (BSL-1)
References: 1.Cohen, A., et al., Cancer Research. 2006 May; 66(9): 4904-4912.2.Scumpia, P.O., et al., Blood. 2007 Oct; 110(10):3673-3681.