Anti-HA-Tag, Monoclonal

Anti-HA-Tag, Monoclonal
Artikelnummer
BPS25010
Verpackungseinheit
100 µg
Hersteller
BPS Bioscience

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Products from BPS Bioscience require a minimum order value above 400€

Applications: WB, DB, ELISA, IP, ICC

Background: Hemagglutinin is an antigenic glycoprotein found on the surface of the influenza viruses, as well as many other bacteria and viruses. It is responsible for binding the virus to the cell that is being infected. The name hemagglutinin comes from the protein's ability to cause red blood cells (erythrocytes) to agglutinate in vitro. The HA tag is derived from the HA molecule corresponding to a.a. 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Clone Number: clone HA.C5/C10

Cross Reactivity: Works in human cells; predicted to work in all proteins containing the HA tag, regardless of species.

Description: Affinity purified anti-HA-tag clone HA.C5/C10, mouse IgG3. Recognizes the N-terminal, C-terminal or internal HA- tagged fusion proteins. Applications: Western Blot (1:1000), Dot Blot, ELISA, Immunocytochemistry (1:200), Immunoprecipitation (1:200), In Situ Hybridisation (1:500-2000). Optimal dilutions/concentrations should be determined by the end user.

Format: Aqueous buffer solution

Formulation: 1 mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN3

Immunogen: Synthetic peptide from influenza hemagglutinin epitope: YPYDVPDYA conjugated to KLH.

Purification: Affinity chromatography

Storage Stability: Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)
Mehr Informationen
Artikelnummer BPS25010
Hersteller BPS Bioscience
Hersteller Artikelnummer 25010
Green Labware Nein
Verpackungseinheit 100 µg
Mengeneinheit STK
Klonalität Monoclonal
Methode Immunoprecipitation, Western Blotting, ELISA, Immunocytochemistry, Dot Blot
Isotyp IgG3
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