CloneID: 3D12
Antigen Long Description: The original antibody was generated by immunizing HLA-B27 transgenic mice with recombinant human HLA-E purified from AEH cells (Lymphoblastoid cell line LCL 721.221 cells transfected with HLA-E gene).
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P13747; P01889; P10321
Specificity Statement: This antibody binds an extracellular epitope on the human HLA-E, a member of human leukocyte antigen family. This antibody is capable of reacting with free HLA-E heavy chain as well as heavy chain associated with b2-microglobulin and peptide. This antibody is reported to cross react with HLA-B and HLA-C but not with HLA-A, HLA-F or HLA-G. The human HLA-E is a non-classical MHC class 1B molecule involved in immune self-nonself discrimination. HLA-E plays a very important role in cell recognition by NK cells. Peptide-bound HLA-E-B2M heterotrimeric complex primarily functions as a ligand for natural killer (NK) cell inhibitory receptor KLRD1-KLRC1, enabling NK cells to monitor the expression of other MHC class I molecules in healthy cells and to tolerate self.
Application Notes (Clone): The biotin conjugated version of this antibody was used for the study of HLA-E expression and distribution on PBMCs using flow cytometry. This antibody was also used in an assay to study the effect of HLA-E on inhibition of NKL-mediated cytolysis LCL .221 cell line. The presence of 3D11 the resulted in complete restoration of lysis (PMID: 9560253). It was reported that binding of this antibody to HLA-E is inhibited by the peptides sequences 115QFAYDGKDY123 and 137DTAAQI142. It was also reported that this antibody is capable of binding several HLA-Ia antigens like HLA-B and HLA-C (PMID: 21145594). This antibody was used in a study to determined the surface expression of HLA-E in human tumors using immunofluorescent staining and flow cytometry (PMID: 12618909).
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