CloneID: YWHG-5
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with synthetic human HLA-G molecule heavy chain α3 structural domain and HLA-G5/6 molecule specific transmembrane region amino acid sequence 'EGLPEPLMLRWSKEGDGGIMS'.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P17693
Specificity Statement: This antibody specifically binds the amino acid sequence 'EGLPEPLMLRWSKEGDGGIMS' in the α3 structural domain of the human HLA-G5 and HLA-G6. HLA-G5 and HLA-G6 are secreted forms of HLA-G that are non-classical major histocompatibility class Ib molecules involved in immune regulatory processes at the maternal-fetal interface. HLA-G expression has been reported in numerous types of cancer, always associated with more advanced stage and aggressive development of the tumor.
Application Notes (Clone): This antibody can bind human HLA-G peptide 'EGLPEPLMLRWSKEGDGGIMS' in an ELISA with a binding affinity of Ka= 1.01 X 10^(9) L/mol. This antibody was capable of detecting G5 and G6 isoforms of human HLA-G in a western blot and does not cross react with any other isoforms of HLA-G. This antibody was also used in the detection of soluble HLA-G5 and HLA-G6 using intracellular flow cytometry of K562-HLA-G5 and K562-HLA-G6 cells. This antibody can also be used for the immunohistochemical staining of HLA-G5/6 molecular expression in gastric cancer tissue using immunohistochemistry (CN114605543).
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