CloneID: scFv-C4
Heavy Chain modification: ScFv
Antigen Long Description: The original antibody was isolated screening a naïve human single-chain Fv antibody phage-display library against HTT(1-17).
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted human scFv antibody, based on the original human scFv format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P42858
Specificity Statement: The antibody is specific for huntingtin. It binds to the first 17 residues of huntingtin [HTT(1-17)].
Application Notes (Clone): The original format of the antibody could inhibit the aggregation of mHTT-exon1 protein fragments in vitro. The crystal structure of the antibody in complex with HTT(1-17) was determined (De Genst et al., 2015; PMID: 25861763). The specificity of the antibody was confirmed by ELISA analysis. The original format of the antibody-targeted the HD-Q103-myc antigen to cell nuclei in COS-7 cells by immunofluorescence microscopy. The binding affinity of the antibody to HTT(1-17) was measured by surface plasmon resonance (Kd= 7.9 nM) (Lecerf et al., 2001; PMID: 11296304). The antibody selectively bound diffuse htt, but not aggregated htt, as shown by immunofluorescence analysis. Immunoprecipitation experiments in stably transfected, intrabody-expressing STHdhQ111 cells demonstrated that the antibody bound a small fraction of wild-type and mutant endogenous full-length htt (Miller et al., 2005; PMID: 15837560).
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