Anti-IGFBP10 (093G9)

Anti-IGFBP10 [093G9], Recombinant, IgG1 kappa, Human

CloneID: 093G9

Heavy Chain modification: Fc Silent™

Antigen Long Description: The original antibody was generated by immunizing mice with human Cyr61.

Buffer Composition: PBS only.

Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.

Uniprot Accession No.: O00622

Specificity Statement: This antibody binds an epitope comprising amino acids 77 to 80, which lie in the C‐terminus of the IGFBP domain of CCN1 (Cyr61). A cyclic peptide encompassing residues 75 to 81 of CCN1 displays high binding affinity for 093G9 (PMID: 28608634). CYR61 is a secreted signaling molecule that promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. This molecule has been implicated as an important mediator in proliferation and metastasis of breast cancer, which indicated that blockage of Cyr61 might be a potent target for breast cancer treatment. Apart from cancer this molecule also plays a role in inflammation, liver fibrosis, and even autoimmune diseases.

Application Notes (Clone): This antibody can bind recombinant human Cyr61 (hCyr61) in an indirect ELISA. In a Biacore assay the binding affinity of this antibody for human Cyr61 was assessed. The dissociation constant for the original mouse IgG1 version was reported to be Kd=6.2nM. This antibody could also bind human Cyr61 extracted from MDA-MB-231 cells and rhCyr61 in a western blot. It can also be used to detect natural Cyr61 protein in cells using immunofluorescence assay. This antibody further revealed inhibitory effects on MDA-MB-231 cell proliferation, migration, and invasion through downregulation of both AKT and ERK phosphorylation in vitro. It also showed significant efficacy on suppressing primary tumor growth and spontaneous lymph node metastasis in in vivo mouse model (PMID: 22048717). Blocking of Cyr61 using this antibody in Sjogren's syndrome(SS)-like model mice could partially alleviate the inflammation and structure disruption of the SMG in this disease, indicating Cyr61 indeed aggravates inflammatory reaction in vivo. Blocking Cyr61 also improved saliva secretion by ameliorating inflammatory infiltration and cytokines production in vivo (PMID: 26630293). It was reported that blocking of CCN1 (Cyr61) resulted in substantial decrease in IL-1b production by keratinocytes in 093G9-treated cells (PMID: 28266627). This antibody could also block the effect of Cyr61 on IL-8 production by fibroblast-like synoviocytes (FLS) of rheumatoid arthritis patients (PMID: 24517278).

• Artikelnummer: ABAAb04655-10.3-BT
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab04655-10.3-BT
• Verpackungseinheit: 1 mg
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Recombinant
• Methode: Immunofluorescence, Western Blotting, ELISA, Blocking, In Vivo Assay
• Isotyp: IgG1 kappa
• Wirt: Human