Anti-IL-2RA (CD25) Antagonist Antibody

Products from BPS Bioscience require a minimum order value above 400€

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Functional validation: The antibody was validated by measuring Anti-IL-2RA binding to IL2RA antigen in ELISA assay. The IL2RA protein (BPS Bioscience #101203) was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified Anti-IL-2RA Antibody (BPS Bioscience #101593), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Goat Anti-Human IgG Fc (HRP) (Abcam #ab97225), washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Description: Recombinant monoclonal anti-CD25 (Basiliximab) which recognizes interleukin-2 receptor alpha subunit (IL-2RA). This affinity purified antibody has been tested for specific binding to purified human IL2RA protein (BPS Bioscience #101203) in a colorimetric ELISA binding assay.

Format: Aqueous buffer solution

Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Host Cell Line: HEK293

Purity: ≥90%

Storage Stability: At least 12 months at -80°C.

Uniprot: P01589

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101593
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101593
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK
• Klonalität: Monoclonal
• Wirt: Human