English
Anti-MeCP2 Polyclonal Antibody
Anti-MeCP2 Polyclonal Antibody
Artikelnummer
BPS25304
Verpackungseinheit
50 µg
Hersteller
BPS Bioscience
Verfügbarkeit:
wird geladen...
Preis wird geladen...
Products from BPS Bioscience require a minimum order value above 400€
Applications: ChIP (5 µg/ChIP)
ELISA (1:1000)
WB (1:1000)
Assay Conditions: ChIP results obtained with the antibody directed against MeCP2 ChIP assays were performed using human osteosarcoma (U2OS) cells, the antibody against MeCP2 (Cat. # 25304) and optimized PCR primer sets. Sheared chromatin from 1x10^6 cells and 5 µg of antibody were used per ChIP experiment. IgG (1 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ZMYND10 gene (used as a positive control) and CDC6 gene (used as a negative control). Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against MeCP2 (Cat. # 25304) and the crude serum. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be: 1:32,900.Western blot analysis using the antibody directed against MeCP2
Nuclear extracts (40 µg) from HeLa cells were analyzed by Western blot using the antibody against MeCP2 (Cat. # 25304) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Background: MeCP2 (UniProt/Swiss-Prot entry P51608) is a chromosomal protein with abundant binding sites in the chromatin. It belongs to the family of methyl CpG binding proteins which also comprises MBD1, MBD2, MBD3 and MBD4. MeCP2 can bind specifically to methylated promoters, thereby repressing transcription. This transcriptional repression is mediated through interaction with histone deacetylase and the corepressor SIN3A. MeCP2 also is essential for development. Mutations in MeCP2 are the cause of several types of mental retardation including Rett syndrome, a progressive neurological disorder that causes mental retardation in females and mental retardation syndromic X-linked type 13, and may also be involved in Angelman syndrome and susceptibility to some types of autism.
Concentration: 50 µg/42 µl
Description: Polyclonal antibody raised in rabbit against MeCP2 (Methyl-CpG-binding domain protein 2), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein
Format: Aqueous buffer solution
Formulation: PBS containing 0.05% azide and 0.05% ProClin 300.
Immunogen: synthetic peptide
Purification: Affinity purified
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Uniprot: P51608
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
Applications: ChIP (5 µg/ChIP)
ELISA (1:1000)
WB (1:1000)
Assay Conditions: ChIP results obtained with the antibody directed against MeCP2 ChIP assays were performed using human osteosarcoma (U2OS) cells, the antibody against MeCP2 (Cat. # 25304) and optimized PCR primer sets. Sheared chromatin from 1x10^6 cells and 5 µg of antibody were used per ChIP experiment. IgG (1 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ZMYND10 gene (used as a positive control) and CDC6 gene (used as a negative control). Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against MeCP2 (Cat. # 25304) and the crude serum. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be: 1:32,900.Western blot analysis using the antibody directed against MeCP2
Nuclear extracts (40 µg) from HeLa cells were analyzed by Western blot using the antibody against MeCP2 (Cat. # 25304) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Background: MeCP2 (UniProt/Swiss-Prot entry P51608) is a chromosomal protein with abundant binding sites in the chromatin. It belongs to the family of methyl CpG binding proteins which also comprises MBD1, MBD2, MBD3 and MBD4. MeCP2 can bind specifically to methylated promoters, thereby repressing transcription. This transcriptional repression is mediated through interaction with histone deacetylase and the corepressor SIN3A. MeCP2 also is essential for development. Mutations in MeCP2 are the cause of several types of mental retardation including Rett syndrome, a progressive neurological disorder that causes mental retardation in females and mental retardation syndromic X-linked type 13, and may also be involved in Angelman syndrome and susceptibility to some types of autism.
Concentration: 50 µg/42 µl
Description: Polyclonal antibody raised in rabbit against MeCP2 (Methyl-CpG-binding domain protein 2), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein
Format: Aqueous buffer solution
Formulation: PBS containing 0.05% azide and 0.05% ProClin 300.
Immunogen: synthetic peptide
Purification: Affinity purified
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Uniprot: P51608
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS25304 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 25304 |
| Green Labware | Nein |
| Verpackungseinheit | 50 µg |
| Mengeneinheit | STK |
| Reaktivität | Human |
| Klonalität | Polyclonal |
| Methode | Immunoprecipitation, Western Blotting, ELISA, Chromatin Immunoprecipitation (ChIP) |
| Produktinformation (PDF) |
|
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
