Anti-Mouse CD27 (RM27-3E5) In Vivo Antibody - Low Endotoxin

Anti-Mouse CD27 (RM27-3E5) In Vivo Antibody - Low Endotoxin, Host: Rat, Rat IgG1 Kappa
Artikelnummer
ICH1185-5MG
Verpackungseinheit
5mg
Hersteller
Ichorbio

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Target: CD27

Clone: RM27-3E5

Specificity: RM27-3E5 activity is directed against mouse CD27

Purification method: This monoclonal antibody was purified using Protein G

Antigen Distribution: CD27 is expressed on T cells, B cells, and NK cells

Background: CD27 is a glycosylated, type I transmembrane protein of the TNFR superfamily that is constitutively expressed on the surface of progenitor and naïve T cells as well as subsets of natural killer (NK) and B cells. CD27 is an immune-checkpoint receptor and ligation with CD70 results in co-stimulatory effects that enhance primary, memory, and recall T cell responses. CD27 signaling has been targeted for the development of viral, autoimmune, and anti-tumor immunotherapies.RM27-3E5 (rat IgG2a) was raised against recombinant mouse CD27-human IgG1 Fc fusion protein and purified from ascites using protein G columns. RM27-3E5 enhances anti-CD3-stimulated T cell proliferation when immobilized in vitro and augments ovalbumin-specific cytotoxic T lymphocyte (CTL) induction when administered in vivo.RM27-3E5 is an agonistic antibody and has been found to support the control of both established lung metastases and subcutaneous tumors. An anti-tumor effect was also observed with RM27-3E5 against EG7/CD27(+) and EG7/CD27(−) tumors, with the effect mediated mainly by T cells with a minor contribution by NK cells. RM27-3E5 exerts its potent anti-tumor effect by inducing a tumor-specific CTL response. RM27-3E5 has potential utility as a non-depleting agonistic anti-CD27 mAb to enhance anti-tumor immunity

Immunogen: Mouse CD27-human IgG1 Fc fusion protein

Concentration: ≥ 5.0 mg/ml

Formulation: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Purity: ≥95% monomer by analytical SEC, >95% by SDS Page

Endotoxin: <1.0 EU/mg as determined by the LAL method

Aggregation: Aggregation level ≤ 5%

Use: Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Mehr Informationen
Artikelnummer ICH1185-5MG
Hersteller Ichorbio
Hersteller Artikelnummer ICH1185-5MG
Verpackungseinheit 5mg
Mengeneinheit STK
Reaktivität Mouse (Murine)
Klonalität Monoclonal
Methode Immunoprecipitation, Flow Cytometry, Functional Assay
Isotyp Rat IgG1 kappa
Wirt Rat
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