Anti-PD-1 (RMP1-30)

Anti-PD-1 [RMP1-30], Recombinant, IgG2b kappa, Mouse

CloneID: RMP1-30

Heavy Chain modification: Fc Silent™

Antigen Long Description: The original antibody was generated by immunizing rats with mouse PD-1 transfectants.

Buffer Composition: PBS with 0.02% Proclin 300.

Chimeric Use Statement: This is a reformatted mouse IgG2b Fc Silent™ antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Uniprot Accession No.: Q02242

Specificity Statement: This antibody is specific for mouse PD-1.

Application Notes (Clone): The original version of this antibody (rat IgG2b) was used to investigate PD-1 signaling pathways in the context of allergic sensitization and challenge in mice. Specifically, it was used to determine how PD-1 blockade affects airway reactivity and immune responses (Matsumoto et al., 2004; PMID: 14764726). The original antibody was used successfully for blocking the PD-1 pathway in combination with ex vivo TKD/IL-2-stimulated NK cells in preclinical models of glioblastoma and lung cancer. It was found that this combination therapy significantly enhanced the cytotoxic activity of NK cells compared to monotherapy. The treatment resulted in improved overall survival, massive infiltration of tumors by CD8⁺ cytotoxic lymphocytes and NK1.1 cells, and a reduction of PD-1⁺ immune cells in the tumor microenvironment. Additionally, the combined therapy maintained its anti-tumor effects even after discontinuation, suggesting long-term benefits and highlighting the potential of this combined immunotherapeutic approach (Shevtsov et al., 2019; PMID: 30967859). The original version of this antibody was used successfully in an in vitro blocking assay on EL4 cells. It was also used for FC analysis and was found to stain the cells with medium intensity compared to the other tested antibodies (Polesso et al., 2021; PMID: 33684223). This antibody was also used for the FC and FACS analyses on tumor-infiltrating immune cells (TIIs) (Yokota et al., 2021; PMID: 34355173) and murine B16-F10 and YUMM melanoma cells and T-cells subsets (Martins et al., 2022; PMID: 35864188). This antibody was tested for its binding affinity to Jurkat cells expressing mouse PD-1. It exhibited intermediate avidity for PD-1 and showed limited capability to reverse PD-1-mediated T-cell inhibition compared to other tested antibodies. It demonstrated partial epitope blocking against itself and another PD-1 monoclonal antibody, indicating its suitability for specific experimental conditions requiring PD-1 staining alongside functional assessments (Bu et al., 2022; PMID: 35925787).

• Artikelnummer: ABAAb04634-3.3
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab04634-3.3
• Verpackungseinheit: 100 μg
• Mengeneinheit: STK
• Reaktivität: Mouse (Murine)
• Klonalität: Recombinant
• Methode: Flow Cytometry, Blocking, In Vivo Assay, Fluorescence-Activated Cell Sorting (FACS)
• Isotyp: IgG2b kappa
• Wirt: Mouse