CloneID: TPL004_01_A11
Heavy Chain modification: Fc Silenced & half-life extended
Antigen Long Description: The antibody was raised using IONTAS phage display library, a naïve human scFv library consisting of 4.0 · 10^10 unique antibody fragments. Through phage display selection, scFv-presenting phages were allowed to bind the venom proteins, phages with unspecific binding were washed away, and the rest were eluted through enzymatic digestion. These were then amplified and used for the following round. In total three rounds of selections were completed leaving the best binders behind.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P00605
Specificity Statement: TPL004_01_A11 antibody recognizes PLA2 (Phospholipase A2 'basic'), which is one of the major proteins in the venom of the black-necked spitting cobra (Naja nigricollis).
Application Notes (Clone): TPL004_01_A11 antibody has a very high affinity to the PLA2. It was demonstrated by normalized ELISA, which circumvents the limitation of a simple ELISA by removing the expression variable, normalizing the amount of scFv in each well. The antibody's high affinity was confirmed by its ability to bind the target protein in a venom fraction even at 1:32,000 dilution (3.125 · 10^-2) µg/mL (Jürgensen et al., 2019 [poster]). It is recommended for the research on the antitoxin against Naja nigricollis snakebites.
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