Anti-ST2L (STLM62 (C2494))

Anti-ST2L [STLM62 (C2494)], Recombinant, IgG2b kappa, Mouse

CloneID: STLM62 (C2494)

Antigen Long Description: The original antibody C2494 was generated by immunizing BALB/c mice with human ST2L-Fc fusion protein. Later on the mouse antibody was converted to human IgG2 chimeric antibody (STLM62).

Buffer Composition: PBS with 0.02% Proclin 300.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Uniprot Accession No.: Q01638

Specificity Statement: This antibody specifically binds an epitope in domain I of the human ST2L and cross reacts with cynomolgus monkey ST2L. ST2L a transmembrane receptor that belongs to the IL-1 receptor family which is expressed on the cell surface of a wide variety of immune cells including T cell, NK/NKT cells, basophils, eosinophils, mast cells and the newly-described non-B/non-T innate lymphoid type 2 cells, nuocytes, and natural helper cells. ST2L expression is also inducible on dendritic cells (DCs), macrophages, and neutrophils. ST2L is able to downregulate the responsiveness of Toll-like Receptors TLR2, TLR4, and TLR9, but also induce type 2 cytokine release via activation by its ligand IL-33 and association with accessory protein IL-1RAcP.

Application Notes (Clone): This antibody binds human ST2L and blocks the interaction between IL-33 and ST2L. This antibody was capable of recognizing recombinant human ST2L extracellular domain (ST2L-ECD) using an ELISA assay (PMID: 27294560). The original human IgG2 chimeric version of the antibody binds human and cynomolgus monkey ST2L with a binding affinity of 28 pM and 12 pM respectively (US9090694). This antibody was capable of binding ST2L expressed by LAD2 mast cells in a flow cytometric assay. This antibody was reported to block the IL-33 induced activation of NF-kB in a dose dependent manner. This antibody also blocked IL-1RAcP from binding to the ST2L/IL-33 complex. It can also inhibit cytokine secretion by human basophilic cell line KU812 (PMID: 27294560).

• Artikelnummer: ABAAb04660-3.0
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab04660-3.0
• Verpackungseinheit: 100 μg
• Mengeneinheit: STK
• Reaktivität: Human, Cynomolgus Monkey (Primate)
• Klonalität: Recombinant
• Methode: ELISA, Flow Cytometry, Blocking, Functional Assay
• Isotyp: IgG2b kappa
• Wirt: Mouse