English
Anti-Sulphonamide drug (4D11)
Anti-Sulphonamide drug [4D11], Recombinant, IgG1 kappa, Human
Artikelnummer
ABAAb04626-10.0-BT
Verpackungseinheit
1 mg
Hersteller
Absolute Antibody
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CloneID: 4D11
Antigen Long Description: The original antibody was generated by immunizing mice with a hapten made by covalently conjugating 4-(4-(4-aminophenylsulfonamido)phenyl)butanoic acid (SA10) with BSA.
Buffer Composition: PBS only.
Specificity Statement: This antibody shows high sensitivity for detection of sulfadoxine (SDM), sulfamonomethoxine (SMM), sulfamethoxazole (SMX), sulfamethazine (SMZ), and sulfaquinoxaline (SQX). It had high affinity for sulfisomidine (SIM), SMZ (including acetyl-SMZ), and sulfanitran (SNT). This antibody has higher affinity for the sulfonamides containing a six-atom-ring substitution (except sulfabenzamide -SBA and SDM) rather than a five atom-ring substitution at the N1 position for e.g. sulfamethizole (SMT) and sulfathiazole (STZ). Sulfonamides are synthetic bacteriostatic antibiotics that competitively inhibit conversion of p-aminobenzoic acid to dihydropteroate, which bacteria need for folate synthesis and ultimately purine and DNA synthesis.
Application Notes (Clone): The binding reactivity of this antibody for various sulphonamide drugs was determined using ELISA (PMID: 23417550). The binding affinity of this antibody towards various sulphonamide drugs is listed in table 1 of PMID: 30580515. This antibody was used in the development of broad-specific and sensitive competitive indirect ELISA for screening for sulfonamides. It used MAb 4D11 and the BS-BSA as heterologous-coating antigen and demonstrated a 50 % specific binding (IC50) for 22 sulfonamides at concentrations below 100 ng mL−1. The optimized ciELISA was used to quantify the five sulfonamides, SMZ, SDM, SQX, SMM, and SMX in spiked milk samples (Lian et al., Food Anal. Methods (2014)).
Antigen Long Description: The original antibody was generated by immunizing mice with a hapten made by covalently conjugating 4-(4-(4-aminophenylsulfonamido)phenyl)butanoic acid (SA10) with BSA.
Buffer Composition: PBS only.
Specificity Statement: This antibody shows high sensitivity for detection of sulfadoxine (SDM), sulfamonomethoxine (SMM), sulfamethoxazole (SMX), sulfamethazine (SMZ), and sulfaquinoxaline (SQX). It had high affinity for sulfisomidine (SIM), SMZ (including acetyl-SMZ), and sulfanitran (SNT). This antibody has higher affinity for the sulfonamides containing a six-atom-ring substitution (except sulfabenzamide -SBA and SDM) rather than a five atom-ring substitution at the N1 position for e.g. sulfamethizole (SMT) and sulfathiazole (STZ). Sulfonamides are synthetic bacteriostatic antibiotics that competitively inhibit conversion of p-aminobenzoic acid to dihydropteroate, which bacteria need for folate synthesis and ultimately purine and DNA synthesis.
Application Notes (Clone): The binding reactivity of this antibody for various sulphonamide drugs was determined using ELISA (PMID: 23417550). The binding affinity of this antibody towards various sulphonamide drugs is listed in table 1 of PMID: 30580515. This antibody was used in the development of broad-specific and sensitive competitive indirect ELISA for screening for sulfonamides. It used MAb 4D11 and the BS-BSA as heterologous-coating antigen and demonstrated a 50 % specific binding (IC50) for 22 sulfonamides at concentrations below 100 ng mL−1. The optimized ciELISA was used to quantify the five sulfonamides, SMZ, SDM, SQX, SMM, and SMX in spiked milk samples (Lian et al., Food Anal. Methods (2014)).
| Artikelnummer | ABAAb04626-10.0-BT |
|---|---|
| Hersteller | Absolute Antibody |
| Hersteller Artikelnummer | Ab04626-10.0-BT |
| Verpackungseinheit | 1 mg |
| Mengeneinheit | STK |
| Reaktivität | Various species |
| Klonalität | Recombinant |
| Methode | ELISA |
| Isotyp | IgG1 kappa |
| Wirt | Human |
| Produktinformation (PDF) |
|
| MSDS (PDF) | Download |
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