Anti-TFR Antibody, Biotin-Labeled

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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: An ELISA binding assay was performed with this antibody and its target protein, TFR1 (BPS Bioscience #101287). The target protein was coated onto an assay plate overnight at 2 µg/ml at 4°. The next day, the plate was washed on blocked. Then, serial dilutions of the antibody were added (0 to 200 nM) for 1 hour at room temperature. After washing, HRP-Streptavidin was incubated for 30 minutes at room temperature. Following another wash step, colorimetric HRP substrate was added and quenched with 1N HCL. Then we measured absorbance on a plate reader at 450 nm. EC50 = 0.61 nM

Description: Recombinant anti-TFR (Transferrin Receptor), which recognizes TFR. The purified antibody was enzymatically biotinylated using Avi-tag^TM technology. This affinity purified antibody has been tested for specific binding to purified human TFR1 (BPS Bioscience #101287) in an ELISA binding assay.

Format: Aqueous buffer solution

Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Host Cell Line: HEK293

Purity: ≥90%

Storage Stability: At least 12 months at -80°C

Uniprot: P02786

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101596
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101596
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK
• Klonalität: Monoclonal
• Wirt: Human