Anti-THC (T3)

Anti-THC [T3], Recombinant, IgG1 kappa, Mouse

CloneID: T3

Antigen Long Description: The original antibody was generated by immunizing mice a THC-BSA conjugate.

Origin Pub PMID: 20630472

Buffer Composition: PBS with 0.02% Proclin 300.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Specificity Statement: This antibody is specific for Δ9-THC and its metabolites, including 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (11-nor-Δ9-THC—COOH) and 11‑hydroxy-Δ9-tetrahydrocannabinol (11-OH-Δ9-THC).

Application Notes (Clone): The binding affinity and specificity of the original version of this antibody (mouse Fab) were determined in a competitive ELISA. Structural analysis of this antibody — free and in complex with THC — was determined through crystallization. This antibody was also used to develop a sensitive, one-step, homogeneous FRET immunoassay for detecting THC and its metabolites in saliva samples (Niemi et al., 2010; PMID: 20630472) (US8518653B2). This antibody was successfully transiently expressed in N. benthamiana by using a geminiviral expression system. The plant-produced version of this antibody retained the binding activity to Δ9-THC and its metabolites and provided high sensitivity in detecting Δ9-THC in marijuana, inflorescence, and leaf extracts but not in stem, root, or non-cannabis controls (Boonyayothin et al., 2022; PMID: 35686006).

• Artikelnummer: ABAAb04635-1.1
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab04635-1.1
• Verpackungseinheit: 100 μg
• Mengeneinheit: STK
• Reaktivität: Various species
• Klonalität: Recombinant
• Methode: ELISA, Functional Assay
• Isotyp: IgG1 kappa
• Wirt: Mouse