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Anti-WDR5 Polyclonal Antibody
Anti-WDR5 Polyclonal Antibody
Artikelnummer
BPS25321
Verpackungseinheit
100 µl
Hersteller
BPS Bioscience
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Applications: ELISA (1:100 - 1:500)
WB (1:1000)
Assay Conditions: Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse Wdr5 (Cat. # 25321). By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:12,300.Western blot analysis using the antibody directed against Wdr5
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Wdr5 (Cat. # 25321), diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (36 kDa) is indicated on the right.
Background: Wdr5 (UniProtKB/Swiss-Prot entry P61964) belongs to the family of WD repeat proteins which are involved in different cellular processes such as cell cycle progression, signal transduction, apoptosis, and gene regulation. It is a component of the Set1A and Set1B histone H3 methylation complexes. These complexes methylate lysine 4 of H3, thereby activating gene transcription. Wdr5 interacts with H3 dimethyl K4, but not with tri or mono methylated H3K4.
Concentration: 100 µl
Description: Polyclonal antibody raised in rabbit against mouse Wdr5 (WD (tryptophan-aspartate) repeat domain protein 5), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein
Format: Aqueous solution
Formulation: Whole antiserum from rabbit containing 0.05% azide
Immunogen: synthetic peptide
Purification: Whole serum
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Uniprot: P61964
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
Applications: ELISA (1:100 - 1:500)
WB (1:1000)
Assay Conditions: Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse Wdr5 (Cat. # 25321). By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:12,300.Western blot analysis using the antibody directed against Wdr5
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Wdr5 (Cat. # 25321), diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (36 kDa) is indicated on the right.
Background: Wdr5 (UniProtKB/Swiss-Prot entry P61964) belongs to the family of WD repeat proteins which are involved in different cellular processes such as cell cycle progression, signal transduction, apoptosis, and gene regulation. It is a component of the Set1A and Set1B histone H3 methylation complexes. These complexes methylate lysine 4 of H3, thereby activating gene transcription. Wdr5 interacts with H3 dimethyl K4, but not with tri or mono methylated H3K4.
Concentration: 100 µl
Description: Polyclonal antibody raised in rabbit against mouse Wdr5 (WD (tryptophan-aspartate) repeat domain protein 5), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein
Format: Aqueous solution
Formulation: Whole antiserum from rabbit containing 0.05% azide
Immunogen: synthetic peptide
Purification: Whole serum
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Uniprot: P61964
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
| Artikelnummer | BPS25321 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 25321 |
| Green Labware | Nein |
| Verpackungseinheit | 100 µl |
| Mengeneinheit | STK |
| Reaktivität | Mouse (Murine) |
| Klonalität | Polyclonal |
| Methode | Western Blotting, ELISA |
| Produktinformation (PDF) |
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| MSDS (PDF) |
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Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
