APC2 Antibody

Application Note: This antibody reacts with human APC2 by western blot and immunoprecipitation.  The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others).  Coimmunoprecipitation of related proteins (APC11) does occur.  A 93.8 kDa band corresponding to human APC2 is detected. Most cell lines or tissues expressing APC2 can be used as a positive control.  Researchers should determine optimal titers for other applications.

Concentration Value: 85 mg/mL

ELISA Dilution: 1:2,000 - 1:10,000

Immunohistochemistry Dilution: User Optimized

Western Blot Dilution: 1:500 - 1:1,000

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 810-822 of Human APC2 (C-terminal) coupled to KLH.

Physical State: Liquid (sterile filtered)

Purity and Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human APC2. Cross reactivity may also occur with APC2 from other sources.  Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and ROC2.

Background: APC2, also known as Anaphase promoting complex subunit 2, APC2, Cyclosome subunit 2, and ANAPC2, is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G₁ phase of the cell cycle.  The APC/cyclosome protein complex promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitors, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains at least eleven subunits. The composition of APC is highly conserved in organisms from yeast to humans. APC2 is a cullin family member that interacts through the cullin domain with ANAPC11 and UBCH10.

Low Endotoxin: No

Other: User Optimized

• Artikelnummer: ROC100-401-A16
• Hersteller: Rockland
• Hersteller Artikelnummer: 100-401-A16
• Verpackungseinheit: 100 µL
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Polyclonal
• Isotyp: Antiserum
• Human Gene ID: 29882
• Wirt: Rabbit
• Konjugat: Unconjugated