Ataxin3 (MJD1, SCA3), His-tag Recombinant

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Encompassing Amino Acids: 2-362(end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and Ataxin-3 (0-0.4 ng/µL) in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.

Description: Human Ataxin-3 (ATX3 or ATXN3), also known as Josephin (JOS), Machado-Joseph disease protein 1 (MJD1) or Spinocerebellar ataxia type 3 (SCA3), GenBank Accession No. NM_004993, a.a. 2-362(end) with an N-terminal His-tag, MW=42.1 kDa, expressed in E. coli.

Format: Aqueous buffer solution

Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT.

Genbank: NM_004993

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: P54252

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. do Carmo Costa, M., et al. (2010). PLoS One 5(7):e11728.
2. Jung, J., et al. (2009). Hum Mol Genet. 18(24):4843-52.
3. Macedo-Ribeiro, S., et al. (2009). PLoS One 4(6): e5834.

• Artikelnummer: BPS80399
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80399
• Green Labware: Nein
• Verpackungseinheit: 250 µg
• Mengeneinheit: STK