ATF1 protein-GST fusion

Application Note: ATF1 Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. ATF1 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~ 63kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Concentration Value: 0.2 µg/µL

Western Blot Dilution: User Optimized

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Physical State: Liquid (sterile filtered)

Purity and Specificity: Recombinant full-length human ATF1 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.

Background: ATF1 is a class of transcription factor that stimulates gene expression of several cell growth-related genes through protein kinase A-related cAMP response elements. ATF1 can physically associate with BRCA1 in vitro, in yeast, and in human cells and BRCA1 is implicated in the transcriptional activation of ATF1 target genes, some of which are involved in the transcriptional response to DNA damage (1). Mitogen- and stress-activated protein kinase 1 (MSK1) and MSK2 play a role in the stress-induced phosphorylation of ATF1 in primary embryonic fibroblasts. However, mitogen-induced phosphorylation of ATF1 is greatly reduced (2). ATF1 Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Angiogenesis, Apoptosis/Autophagy, Cardiovascular Disease, Cellular Stress, Inflammation, Metabolic Disorder, Neurobiology, NfkB Pathway, and p38 Pathway research.

Low Endotoxin: No

Other: Kinase Assay-User Optimized

• Artikelnummer: ROC009-001-R42S
• Hersteller: Rockland
• Hersteller Artikelnummer: 009-001-R42S
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Konjugat: Unconjugated