Application Note: Anti-Avi Epitope Tag Antibody has been tested in ELISA and Western blot. Minor background bands of unknown identity may be observed. The antisera will recognize the Avi-tag irrespective of the whether the tag is biotinylated (data not shown).
Concentration Value: 90 mg/ml
ELISA Dilution: 1:5,000
Western Blot Dilution: 1:5,000
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Immunogen: Anti-Avi Epitope Tag antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic avi-tag peptide GLNDIFEAQKIEWH (minimal BirA substrate; biotin is added to the sequence at the lysine).
Physical State: Liquid (sterile filtered)
Purity and Specificity: This antiserum detects the minimal peptide substrate GLNDIFEAQKIEWH for the E. coli biotin ligase BirA. Western blot analysis of B. subtilis strains producing either (Avi-tagged) SPoIIIAH (XWB49) or (Avi-tagged) SpoIIQ (XWB46) showed strong and specific reactivity to the antisera.
Background: The peptide is also called the biotin acceptor peptide (BAP) of the biotin carboxyl carrier protein (BCCP) subunit of acetyl CoA carboxylase of E. coli.
Low Endotoxin: No
Other: User Optimized