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Bas (PTPN13), GST-tag Recombinant
Bas (PTPN13), GST-tag Recombinant
Artikelnummer
BPS30024
Verpackungseinheit
50 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 2091-2490
Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Assay Conditions: The specific activity of Bas was determined using pNPP. Assay conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 20 mM pNPP, and 20 ng/µl enzyme for 5 minutes at 30°C.
Background: PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN13 is a large protein that possesses a PTP domain at C-terminus, and multiple noncatalytic domains, which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains, and a leucine zipper motif. PTPN13 binds to a negative regulatory domain in Fas that inhibits Fas-induced apoptosis.
Description: Human, recombinant PTP-Bas, catalytic domain (Gene bank accession number NM_080685), a.a. 2091-2490 (catalytic domain), with N-terminal GST tag, MW=71.8kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
Genbank: NM_080685
Storage Stability: >12 months at -80°C
Tags: N-terminal GST-tag
Uniprot: Q12923
Unit Definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. W. Kimber, et al., Biochem.J. 2003, 376: 525-535.
2. N. Kachel, et al. J.Mol. Biol. 2003, 334: 143-155.
Encompassing Amino Acids: 2091-2490
Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Assay Conditions: The specific activity of Bas was determined using pNPP. Assay conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 20 mM pNPP, and 20 ng/µl enzyme for 5 minutes at 30°C.
Background: PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN13 is a large protein that possesses a PTP domain at C-terminus, and multiple noncatalytic domains, which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains, and a leucine zipper motif. PTPN13 binds to a negative regulatory domain in Fas that inhibits Fas-induced apoptosis.
Description: Human, recombinant PTP-Bas, catalytic domain (Gene bank accession number NM_080685), a.a. 2091-2490 (catalytic domain), with N-terminal GST tag, MW=71.8kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
Genbank: NM_080685
Storage Stability: >12 months at -80°C
Tags: N-terminal GST-tag
Uniprot: Q12923
Unit Definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. W. Kimber, et al., Biochem.J. 2003, 376: 525-535.
2. N. Kachel, et al. J.Mol. Biol. 2003, 334: 143-155.
| Artikelnummer | BPS30024 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 30024 |
| Green Labware | Nein |
| Verpackungseinheit | 50 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
