BCL2 protein-GST fusion

Application Note: BCL2 Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. BCL2 Protein is suitable for use in Western Blot. Expect a band approximately ~ 51kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Concentration Value: 0.2 ug/ml

Western Blot Dilution: User Optimized

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Physical State: Liquid (sterile filtered)

Purity and Specificity: Recombinant full-length human BCL2 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.

Background: BCL2 gene encodes an integral inner mitochondrial membrane protein that acts as an antiapoptotic protein (1). The protein BAD can antagonize both the cell cycle and antiapoptotic functions of BCL2 through binding to the BH3 domain. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma (2). BCL2 is phosphorylated on specific serine/threonine residues within the unstructured loop in response to diverse stimuli and such phosphorylation has been associated with the loss of the biological function of BCL2. BCL2 Protein is ideal for investigators involved in Signaling Proteins, Apoptosis Proteins, Apoptosis/Autophagy, Cancer, Cellular Stress, and Neurobiology research.

Low Endotoxin: No

• Artikelnummer: ROC009-001-R49S
• Hersteller: Rockland
• Hersteller Artikelnummer: 009-001-R49S
• Verpackungseinheit: 20 µg
• Mengeneinheit: STK
• Konjugat: Unconjugated