BCL2L2, His-Tag Recombinant

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Applications: Useful as a positive control for Western blot studies.

Assay Conditions: Add 20-40 ng of BCL2L2 to 20 µl reaction mix containing 130 nM MCL1 substrate, anti-His Tb-labeled donor, and dyelabeled acceptor. Incubate 1 hour at room temperature. Read TR-FRET (λexc=340 nm, λem1=620 nm and λem2=665 nm).

Description: Human BCL2L2, also known as Bcl-2-like protein 2, and BCL2 like 2, a. a. 1-172, with C-terminal His-tag, GenBank Accession No. NM_004050, MW = 20 kDa, expressed in an E. coli expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3mM DTT and Imidazole

Genbank: NM_004050

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal His-tag

Uniprot: Q92843

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Shi, G. et al., Experimental Brain Research. Nov; 216(2) : 225-230 (2012).2. Qu J. et al., Journal of Cellular Physiology. 230(3): 1868-1878 (2018).

• Artikelnummer: BPS100091-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100091-2
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Wirt: Escherichia Coli