Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to human lysozyme

Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to human lysozyme, Polyclonal, IgG, Clone: Polyclonal
Artikelnummer
RAHu/Lys/Bio
Verpackungseinheit
1 ml
Hersteller
Nordic-MUbio

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Clone: Polyclonal

Label: Biotin

Background: The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum. In immunocytochemical and immunohistochemical techniques for the detection of lysozyme at the cellular and subcellular level in appropriately treated cell and tissue substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Immunogen: Highly purified recombinant human lysozyme was used as immunogen.

Source: Highly purified recombinant human lysozyme. Lysozyme, a bacteriolytic enzyme, has a molecular weight of about 15,000. Its concentration in plasma is low; in milk the concentration is reported to be between 5 and 220 mg/ml. Also tears contain a considerable amount of lysozyme.Freund's complete adjuvant is used in the first step of the immunization procedure.

Specificity: Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to Human lysozyme.

Formulation: Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.

Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Artikelnummer RAHu/Lys/Bio
Hersteller Nordic-MUbio
Hersteller Artikelnummer RAHu/Lys/Bio
Green Labware Nein
Verpackungseinheit 1 ml
Mengeneinheit STK
Reaktivität Human
Klonalität Polyclonal
Methode Immunohistochemistry (frozen), Western Blotting, ELISA, Immunocytochemistry
Isotyp IgG
Wirt Rabbit
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