BirA, GST-Tag (E. coli) (E. coli derived) Recombinant

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Encompassing Amino Acids: 2-321 (end)

Applications: Useful for covalently biotin-labeling of acceptor proteins for detection and purification assays.

Assay Conditions: BirA (0.16 mg/ml) completely biotinylated 10 µM of Avi-tag substrate at 30°C for 30 minutes in reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 µM d-Biotin.

Description: Biotin ligase BirA, GenBank AccessionNo. NP_418404, a.a. 2 - 321 with NterminalGST-tag, MW = 61 kDa,expressed in an E. coli cell expressionsystem.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT, and 20% glycerol.

Genbank: NP_418404

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: P06709

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Li, Y., et al., Protein Expr Purif. 2012 Mar, 82(1): 162-7.
2. Chakravartty, V., et al., J Bacteriol. 2012 Mar, 194(5): 1113-26.

• Artikelnummer: BPS70031
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 70031
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK