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BirA, His-FLAG-tags (E. coli) (E. coli-derived) Recombinant
BirA, His-FLAG-tags (E. coli) (E. coli-derived) Recombinant
Artikelnummer
BPS70030
Verpackungseinheit
100 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 2-321 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: BirA (10 µg/ml) completely biotinylated 10 µM of substrate MBP-Avi at 30°C for 30 minutes in the standard reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 µM d-Biotin.
Description: Biotin ligase BirA, GenBank Accession No. NP_418404, a.a. 2 - 321 with N-terminal His-FLAG tag, MW = 37.1 kDa, expressed in an E. coli cell expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 150 mM imidazole, 3 mM DTT, and 5% glycerol.
Genbank: NP_418404
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-FLAG-tags
Uniprot: H0QFJ5
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Li, Y., et al., Protein Expr Purif. 2012 Mar; 82(1): 162-7.
2. Chakravartty, V., et al., J Bacteriol. 2012 Mar; 194(5): 1113-26.
Encompassing Amino Acids: 2-321 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: BirA (10 µg/ml) completely biotinylated 10 µM of substrate MBP-Avi at 30°C for 30 minutes in the standard reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 µM d-Biotin.
Description: Biotin ligase BirA, GenBank Accession No. NP_418404, a.a. 2 - 321 with N-terminal His-FLAG tag, MW = 37.1 kDa, expressed in an E. coli cell expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 150 mM imidazole, 3 mM DTT, and 5% glycerol.
Genbank: NP_418404
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-FLAG-tags
Uniprot: H0QFJ5
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Li, Y., et al., Protein Expr Purif. 2012 Mar; 82(1): 162-7.
2. Chakravartty, V., et al., J Bacteriol. 2012 Mar; 194(5): 1113-26.
| Artikelnummer | BPS70030 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 70030 |
| Green Labware | Nein |
| Verpackungseinheit | 100 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
