BLK, GST-tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: BLK, also known as B lymphoid kinase, is a 55 kd tyrosine kinases with a SH3, SH2 and catalytic domains that contain consensus sequences of the src protein tyrosine kinase family. BLK is expressed specifically in the B cell lineage and plays a role in signal transduction pathway that is restricted to B lymphoid cells (1). Stimulation of resting B-lymphocytes with antibodies to surface immunoglobulin (sIgD or sIgM) induces activation of BLK (2).

Description: Recombinant full-length human BLK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST-tag. The gene accession number is BC007371.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

Genbank: BC007371

Host Cell Line: Sf9 cells

Purity: ≥90%

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: P51451

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Dymecki, SM. et al. Science. 1990 Jan 19;247(4940):332-6.
2. Burkhardt, AL. et al. Proc Natl Acad Sci USA. 1991 Aug 15;88(16):7410-4.

• Artikelnummer: BPS40401
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40401
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect