BMX, GST-tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was done in Kinase buffer containing 0.2 mM DTT using Poly-(Tyr,Glu) substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was measured using Kinase-Glo reagent (Promega)

Background: The BMX gene encodes a non-receptor tyrosine kinase, which may play a role in the growth and differentiation of hematopoietic cells (1). The BMX gene is located on chromosomal band Xp22.2 between the DXS197 and DXS207 loci. Interestingly, chromosome X also contains the closest relative of BMX, the BTK gene, implicated in X-linked agammaglobulinemia. BMX, is found to induce activation of the Stat signaling pathway (2).

Description: Recombinant full-lengthhuman cytoplasmic tyrosine-proteinkinase BMX (BMX), also known as bonemarrow tyrosine kinase gene inchromosome X protein, epithelial andendothelial tyrosine kinase, ETK, andNTK38 was expressed by baculovirus inSf9 insect cells using an N-terminal GSTtag, a.a. 1-675 (end). The geneaccession number is NM_001721(variant 2).

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25%glycerol

Genbank: NM_001721

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: P51813

Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).

Biosafety Level: Not applicable (BSL-1)

References: 1. Tamagnone, L. et al. Oncogene. 1994 Dec;9(12):3683-8.
2. Saharinen, P. et al. Blood. 1997 Dec 1;90(11):4341-53

• Artikelnummer: BPS40402
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40402
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect