BRD2 (BD2), GST-tag Recombinant

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Encompassing Amino Acids: 339-459

Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Assay Conditions: Assay was performed using BRD2 BD2 Inhibitor Screening Assay Kit, Cat. #32522. BRD2 protein is incubated with biotinylated acetyl-histone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA® GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen® Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.

Description: Human Bromodomain containing 2, also known as BRD2, RING3 or RNF3, GenBank Accession No. NM_005104, a.a. 65 - 187 corresponding to bromodomain 2 with N-terminal GST-tag, MW = 41.4 kDa, expressed in an E. coli expression system.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 10% glycerol, 3 mM EDTA, 20 mM glutathione, and 2 mM DTT

Genbank: NM_005104

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: P25440

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Umehara, T., et al., FEBS Lett. 2010 Sep 24; 584(18): 3901-3908.
2. Shang, E., et al., J Cell Biochem. 2011 Oct; 112(10): 2784-2793.

• Artikelnummer: BPS31023
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 31023
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Wirt: Escherichia Coli