BRD3 (BD1), His-tag Recombinant

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Encompassing Amino Acids: 29-145

Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Assay Conditions: BRD3 is incubated with biotinylated acetyl-histone peptide substrate (BPS #33000) in BRD assay buffer (BPS #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA® Nickel Chelate acceptor beads (PerkinElmer) are added, followed by Alphascreen® Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour minute incubation at RT, Alphacounts are measured.

Description: Human Bromodomain containing 3, also known as BRD3 or RING3L, GenBank Accession No. NM_007371, a.a. 29-145 corresponding to bromodomain 1 with N-terminal His-tag, MW = 14.9 kDa, expressed in an E. coli expression system.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 0.05% Tween-20, and 10% glycerol

Genbank: NM_007371

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: Q15059

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Gamsjaeger, R., et al., Mol Cell Biol. 2011 Jul; 31(13): 2632-2640.
2. Lamonica, JM, et al., Proc Natl Acad Sci USA. 2011 May 31; 108(22): E159-168.

• Artikelnummer: BPS31030
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 31030
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Wirt: Escherichia Coli