BRD4 (BD1+BD2), GST-Tag Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Encompassing Amino Acids: 49-460

Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Assay Conditions: BRD4 protein is incubated with biotinylated acetyl-histone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA® GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen® Streptavidin -conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.

Description: Human Bromodomain containing 4, also known as BRD4, HUNK1, or MCAP, GenBank Accession No. NM_058243, a.a. 49-460 corresponding to bromodomains 1 and 2 with N-terminal GST-tag, MW = 73.3 kDa, expressed in an E. coli expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 20% glycerol and 3 mM DTT.

Genbank: NM_058243

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: O60885

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Rodriguez, RM, et al., J Mol Med (Berl). 2012 May;90(5):587-95.

• Artikelnummer: BPS31044
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 31044
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Wirt: Escherichia Coli