BRD4 (BD2), GST-tag Recombinant

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Encompassing Amino Acids: 342-460

Applications: Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Assay Conditions: Assay was performed using BRD4 Inhibitor Screening Assay Kit, Cat. #32524. BRD4 protein is incubated with biotinylated acetyl-histone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA® GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen® Streptavidin-conjugated donor beads (PerkinElmer). After 15 minute incubation at RT, Alphacounts are measured.

Description: Human Bromodomain containing 4, also known as BRD4, HUNK1, or MCAP, GenBank Accession No. NM_058243, a.a. 342-460 corresponding to bromodomain 2 with N-terminal GST-tag, MW = 40.6 kDa, expressed in an E. coli expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol, and 3 mM DTT

Genbank: NM_058243

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: O60885

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Rodriguez, RM, et al., J Mol Med (Berl). 2012 May;90(5):587-95.
2. Voigt, P, et al., Genome Biol. 2011 Nov29;12(11):133.

• Artikelnummer: BPS31041
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 31041
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Wirt: Escherichia Coli