c-CBL, GST-Tag Recombinant

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Encompassing Amino Acids: 49-434

Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.

Assay Conditions: 10 µL of reaction mixture contains 50 mM Tris, pH 7.4, 5 mM MgCl2, 0.01% Tween, 0.2 µM Ub[Biotin], 50 nM Ube1, 1 mM ATP, 2 µM of UBCH5b, and various amount of CBL-B and C-CBL. The reaction was initiated with the addition of ATP, and incubated at 30°C for 3 hours. Tb labeled anti-GST antibody donor and xl665 labeled acceptor was added and incubated at room temperature for one hour.

Description: Human CBL, also known as CBL-2, Cbl Proto-Oncogene, Signal Transduction Protein CBL, and Cbl Proto-Oncogene, E3 Ubiquitin Protein Ligase, Genbank Accession No. NM_005188, a.a. 49-434 with N-terminal GST-tag, expressed in an E. coli expression system. MW = 71 kDa.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.04% Tween20, 20% glycerol, 16 mM glutathione, 3 mM DTT.

Genbank: NM_005188

Storage Stability: At least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Tags: N-terminal GST-Tag

Uniprot: P22681

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Griffiths, E.K., Molec. and Cellular Bio. 2003; Oct. 23(21):7708-7718Keane, M.M., Oncogene, 1999 June; 18: 3365-3375.

• Artikelnummer: BPS100370
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100370
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK