Calmodulin, His-tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for studying calmodulin-dependent kinase kinetics in vitro.

Assay Conditions: Varying amounts of calmodulin 25 ng CAMK2, 200 ng/µl autocamtide-2 peptide, 0.4 mM CaCl2, 50 µM ATP and trace [33P]--ATP in 25 µl of reaction volume for 15 min at 30°C.

Description: Human Calmodulin 1, alsoknown as CALM1, CALML2, CAMI, DD132,PHKD, GenBank Accession No.NM_006888, full-length with an N-terminalHis-tag, expressed in an E. coli cellexpression system. MW = 17 kDa.

Format: Aqueous buffer solution.

Formulation: 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.

Genbank: NM_006888

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: P62158

Warnings: Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).

Biosafety Level: Not applicable (BSL-1)

References: 1. Li, H. et al., FEBS Lett. 2004; 13(559): 175-180.
2. Huang, M. et al., Cell Stress Chaperones. 2009; 14(4): 343-353.

• Artikelnummer: BPS81152
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 81152
• Green Labware: Nein
• Verpackungseinheit: 50 µg
• Mengeneinheit: STK