CAMK1δ, GST-tag Recombinant

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Encompassing Amino Acids: full length

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Background: CAMK1δ or Ca2+/calmodulin-dependent kinase I-like kinase (CKLiK) is activated by Ca(2+) and calmodulin and is detected in CD34+-derived neutrophils and eosinophils, as well as in mature peripheral blood granulocytes (1). CAMK1δ exhibits Ca(2+)/CaM-dependent activity that is enhanced in vitro by phosphorylation of its Thr180 by CaM-K kinase (CaM-KK)alpha, consistent with detection of CAMK1D-activating activity in HeLa cells (2).

Description: Recombinant human CAMK1δ, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Format: Aqueous buffer solution

Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

Genbank: NM_153498

Host Cell Line: Sf9 cells

Purity: ≥75%

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal GST-tag

Uniprot: Q8IU85

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Verploegen, S. et al: Characterization of the role of CaMKI-like kinase (CKLiK) in human granulocyte function. Blood 106 (3), 1076-1083 (2005).
2. Ishikawa, Y. et al: Identification and characterization of novel components of a Ca2+/calmodulin-dependent protein kinase cascade in HeLa cells. FEBS Lett. 550 (1-3), 57-63 (2003).

• Artikelnummer: BPS40037
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 40037
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK
• Wirt: Insect