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CARP2, SUMO-His-tags Recombinant
CARP2, SUMO-His-tags Recombinant
Artikelnummer
BPS81050
Verpackungseinheit
25 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: full length
Applications: Useful as an E3 ligase in ubiquitin-conjugating reactions and to analyze upregulation/downregulation of E3 enzymes.
Background: CARP2 (caspase 8/10 associated RING protein 2) is a RING-domain E3 (ubiquitin protein ligase) that is involved in the conjugation of ubiquitin to target substrates, along with E1 and E2 enzymes. CARPs (CARP1 and CARP2, 77% identity) also belong to the IAP family (inhibitors of apoptosis proteins) inhibiting activation of DED (death effector domain) containing caspase proteins (8 and 10). In addition to targeting caspases 8 and 10, CARPs have been shown to target phosphorylated p53 for degradation in an Hdm2 independent manner. Furthermore, CARP2 has been shown to be a negative regulator of TNF induced NF-κappaB activation by targeting RIP for degradation.
Biological Activity: Typical enzyme concentration of 100 nM - 5 mM is used for in vitro conjugation, depending on conditions.
Description: Human CARP2 recombinant protein (Genbank Accession No. NP_476519), full length with N-terminal SUMO- and His-tags, expressed in E. coli.
Format: Aqueous buffer solution
Formulation: 50 mM Tris Buffer, pH 7.6, 150 mM NaCl, 10% glycerol
Genbank: NP_476519
Purity: ≥95% by SDS-PAGE
Storage Stability: Stable for≥1 year at -80°C.
Tags: N-terminal SUMO-His-tags
Uniprot: O75493
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yang, W., et al., CARPs are ubiquitin ligases that promote MDM2-independent p53 and phospho-p53ser20 degradation. J Biol Chem, 2007. 282:3273-81.
2. Yang, W. and W.S. El-Deiry, CARPs are E3 ligases that target apical caspases and p53. Cancer Biol Ther, 2007. 6:1676-83.
3. McDonald, E.R., 3rd and W.S. El-Deiry, Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth. Proc Natl Acad Sci USA, 2004. 101:6170-5.
4. Liao, W., et al., CARP-2 is an endosome-associated ubiquitin ligase for RIP and regulates TNF-induced NF-κappaB activation. Curr Biol, 2008. 18:641-9.
Encompassing Amino Acids: full length
Applications: Useful as an E3 ligase in ubiquitin-conjugating reactions and to analyze upregulation/downregulation of E3 enzymes.
Background: CARP2 (caspase 8/10 associated RING protein 2) is a RING-domain E3 (ubiquitin protein ligase) that is involved in the conjugation of ubiquitin to target substrates, along with E1 and E2 enzymes. CARPs (CARP1 and CARP2, 77% identity) also belong to the IAP family (inhibitors of apoptosis proteins) inhibiting activation of DED (death effector domain) containing caspase proteins (8 and 10). In addition to targeting caspases 8 and 10, CARPs have been shown to target phosphorylated p53 for degradation in an Hdm2 independent manner. Furthermore, CARP2 has been shown to be a negative regulator of TNF induced NF-κappaB activation by targeting RIP for degradation.
Biological Activity: Typical enzyme concentration of 100 nM - 5 mM is used for in vitro conjugation, depending on conditions.
Description: Human CARP2 recombinant protein (Genbank Accession No. NP_476519), full length with N-terminal SUMO- and His-tags, expressed in E. coli.
Format: Aqueous buffer solution
Formulation: 50 mM Tris Buffer, pH 7.6, 150 mM NaCl, 10% glycerol
Genbank: NP_476519
Purity: ≥95% by SDS-PAGE
Storage Stability: Stable for≥1 year at -80°C.
Tags: N-terminal SUMO-His-tags
Uniprot: O75493
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yang, W., et al., CARPs are ubiquitin ligases that promote MDM2-independent p53 and phospho-p53ser20 degradation. J Biol Chem, 2007. 282:3273-81.
2. Yang, W. and W.S. El-Deiry, CARPs are E3 ligases that target apical caspases and p53. Cancer Biol Ther, 2007. 6:1676-83.
3. McDonald, E.R., 3rd and W.S. El-Deiry, Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth. Proc Natl Acad Sci USA, 2004. 101:6170-5.
4. Liao, W., et al., CARP-2 is an endosome-associated ubiquitin ligase for RIP and regulates TNF-induced NF-κappaB activation. Curr Biol, 2008. 18:641-9.
| Artikelnummer | BPS81050 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 81050 |
| Green Labware | Nein |
| Verpackungseinheit | 25 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) |
|
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
