Cas9-Expressing Daudi Cell Line

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Applications: 1. Quickly generating knock-out cell pools or cell lines in Daudi cells.
2. Implementing sgRNA screens in Cas9 expressing Daudi cells.

Background: Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing Daudi cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.

Description: This cell line is a clonal derivative from the Cas9-Expressing Daudi Cell Pool (BPS Bioscience, #78089). It was generated by limiting dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing cell line. The expressed Cas9 protein includes a C-terminal FLAG tag.

Host Cell Line: Daudi

Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.

Storage Stability: Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. 

Supplied As: Each vial contains ~2 x 10^6 cells in 1 ml of FBS with 10% DMSO.

Biosafety Level: BSL-1

• Artikelnummer: BPS78157
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78157
• Verpackungseinheit: 2 vials
• Mengeneinheit: PAK
• Wirt: Human