Cas9 Expressing HCT116 Cell Pool

Products from BPS Bioscience require a minimum order value above 400€

Applications: 1. Quickly generating knock-out cell pools or cell lines in HCT116 cells.
2. Implementing sgRNA screens in Cas9-expressing HCT116 cells.

Description: Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing HCT116 cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.

Host Cell Line: HCT116

Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.

Storage Stability: Immediately upon receipt, store in liquid nitrogen.

Supplied As: Each vial contains ~2 x 10^6 cells in 1 ml of FBS with 10% DMSO.

Biosafety Level: BSL-2

• Artikelnummer: BPS78073
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 78073
• Green Labware: Nein
• Verpackungseinheit: 2 vials
• Mengeneinheit: PAK
• Wirt: Human