Caspase-7 Homogeneous Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Great for studying enzyme kinetics and HTS applications.

Background: Caspase-7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis.

Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt

Description: The Homogeneous Caspase-7 Assay Kit is a complete assay system designed to measure Caspase-7 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-7 activity measurements. In addition, the kit includes purified Caspase-7 enzyme and a potent Caspase-3/7 inhibitor, Ac-DNLD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-7 activity level. The fluorogenic substrate, Ac-DEVD-AFC, is incubated with purified Caspase-7 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader.

Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Chai, J., et al., Cell, 2001 Mar 9;104(5):769-80.
2. Denault, J.B., and Salvesen, GS., J. Biol. Chem., 2003 Sep 5;278(36):34042-50.

• Artikelnummer: BPS80701
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80701
• Green Labware: Nein
• Verpackungseinheit: 96 rxns.
• Mengeneinheit: PAK