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Encompassing Amino Acids: 1-277(end)
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done according to BPS Bioscience #80700 with various amounts of Caspase3 (Rat) #102292.
Background: Caspase-3 is a cysteine-aspartic acid protease of the caspase family of proteins, belonging to group II (apoptotic executioner caspases). It plays a role in cell differentiation, tissue regeneration, apoptosis and neural development. It is synthesized as a zymogen, or procaspase, requiring cleavage by initiator caspases to trigger apoptosis. Its activity results in several cell changes, such as membrane blebbing, DNA fragmentation and the formation of apoptotic vesicles. Cell death via apoptosis can occur by intrinsic and extrinsic pathways. In response to DNA damage, metabolic or ER (endoplasmic reticulum) stress the intrinsic pathway can be activated. Caspase-3 can be inhibited by XIAP (X-linked IAP), cIAP1 and cIAP2. Deregulation of caspase activity can result in cancer, as many of the regulators of their activity are oncogenes or tumor suppressors. Caspase-3 has been used in the treatment of cancer, heart failure and neurogenerative disorders. Further understanding of the role of caspase-3 in disease will allow us to develop innovative and efficacious new therapies.
Description: Rat recombinant caspase3 protease, full length encompassing amino acids 1-277 (end). This construct contains a C-terminal His-tag (6xHis). The protein was affinity purified.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT
Genbank: NM_012922.2
Purity: ≥90%
Supplied As: At least 6 months at -80°C.
Tags: Caspase-3, CASP-3, Apopain, Cysteine protease CPP32 (CPP-32), IRP, LICE, Protein Yama, SREBP cleavage activity 1 (SCA-1), Casp3, Cpp32
Target: C-terminal His-Tag
Uniprot: P55213
Warnings: Avoid multiple freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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