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Caspase9, His-tag Recombinant
Caspase9, His-tag Recombinant
Artikelnummer
BPS80115
Verpackungseinheit
50 µg
Hersteller
BPS Bioscience
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Encompassing Amino Acids: 1 - 416 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 mM HEPES, pH 7.2,50 mM NaCl, 10 mM EDTA, 0.1% Chaps,5% glycerol, 10 mM DTT, and 10 µMCaspase 9 substrate. Incubate for 30minutes at room temperature. Fluorescenceintensity is measured at exc380/em505 nm.
Description: Human Caspase 9 (CASP9), GenBank Accession No. NM_001229, a.a. 1 - 416 (end), with C-terminal His-tag, MW=35 &10 kDa, expressed in an E. coli cell expression system. Procaspase-9 is autocleaved between the prodomain and large protease subunit (p35). The cleavage product contains both the large (p35) and small (p10 [His-tag]) protease subunits.
Format: Aqueous buffer solution
Formulation: 50 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT,80 mM imidazole.
Genbank: NM_001229
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P55211
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Guerrero AD., et al., J Clin Cell Immunol.9;3(3) (2012)
2. Luo W., et al., Int J Biol Macromol. S0141-8130(13)00222-5. (2013)
Encompassing Amino Acids: 1 - 416 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 mM HEPES, pH 7.2,50 mM NaCl, 10 mM EDTA, 0.1% Chaps,5% glycerol, 10 mM DTT, and 10 µMCaspase 9 substrate. Incubate for 30minutes at room temperature. Fluorescenceintensity is measured at exc380/em505 nm.
Description: Human Caspase 9 (CASP9), GenBank Accession No. NM_001229, a.a. 1 - 416 (end), with C-terminal His-tag, MW=35 &10 kDa, expressed in an E. coli cell expression system. Procaspase-9 is autocleaved between the prodomain and large protease subunit (p35). The cleavage product contains both the large (p35) and small (p10 [His-tag]) protease subunits.
Format: Aqueous buffer solution
Formulation: 50 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT,80 mM imidazole.
Genbank: NM_001229
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P55211
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Guerrero AD., et al., J Clin Cell Immunol.9;3(3) (2012)
2. Luo W., et al., Int J Biol Macromol. S0141-8130(13)00222-5. (2013)
| Artikelnummer | BPS80115 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 80115 |
| Green Labware | Nein |
| Verpackungseinheit | 50 µg |
| Mengeneinheit | STK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
Immer für Sie da
Gernot Ensinger, M.Sc.
Laurent Haze
