Cathepsin L, His-tag Recombinant

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Encompassing Amino Acids: 18-333

Applications: Useful for activation of DPP1 (Cathepsin C). Suitable for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Assay Conditions: Z-Leu-Arg-AMC (20 µM)was used as a substrate. Reaction wasperformed in a buffer containing 50 mM MES(pH 5.0), 5 mM DTT for 21 min at RT.

Description: Human cathepsin L (GenBank Accession No. NM_001912), a.a. 18-333, with C-terminal His-tag, MW=36 kDa, expressed in FreeStyle 293-F cells.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

Genbank: NM_001912

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal His-tag

Uniprot: P07711

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Jean D. et al., Biochem. J. 413 (1), 125-134 (2008).
2. Abboud-Jarrous G. et al., J. Biol. Chem. 283 (26), 18167-18176 (2008).

• Artikelnummer: BPS80005
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 80005
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK