CBFb Antibody

Application Note: Anti-CBFb Antibody has been tested in ChIP and ELISA. Specific conditions for reactivity should be optimized by the end user.

Concentration Value: neat

ELISA Dilution: 1:500

ChIP Dilution: 4 μl/ChIP

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen: Anti-CBFb Antibody was produced in rabbits by repeated immunizations with human CBFb using two synthetic peptides containing sequences from the internal region of the protein.

Physical State: Liquid (sterile filtered)

Purity and Specificity: Anti-CBFb Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human CBFb.  Cross reactivity with CBFb from other sources is not known.

Background: CBFb represents the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family. These transcription factors regulate a host of genes specific to haematopoiesis (e.g. RUNX1) and osteogenesis (e.g. RUNX2). The beta subunit is the regulatory subunit which allosterically enhances the activity of the DNA binding alpha subunit as the complex binds to the core site of various enhancers and promoters. CBFb can be involved in a chromosomal rearrangement of chromosome 16 (inv(16)(p13q22)) which produces a fusion protein consisting of the N terminus of CBFb and the C-terminal portion of MYH11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Anti-CBFb Antibody is ideal for research in Gene Expression, Cell Differentiation and Cancer.

Low Endotoxin: No

• Artikelnummer: ROC100-401-V32
• Hersteller: Rockland
• Hersteller Artikelnummer: 100-401-V32
• Verpackungseinheit: 100 µL
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Polyclonal
• Methode: ELISA, Chromatin Immunoprecipitation (ChIP)
• Isotyp: Antiserum
• Human Gene ID: 865
• Wirt: Rabbit
• Konjugat: Unconjugated