CD20, FLAG-Tag Recombinant

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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: The protein was validated by measuring its binding to Anti-CD20 antibody, Biotin-labeled (BPS Bioscience #101207) in biochemical binding (ELISA) assay. The CD20 protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) with DDM/CHS detergent (dodecyl-β-D-maltoside (DDM) 0.05%, cholesteryl hemisuccinate (CHS) 0.01%) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified anti-CD20 antibody, biotin-labeled, serially diluted in Blocking Buffer 2 with DDM/CHS detergent, was added for 60 minutes at room temperature. After 3 more washes, the plate was incubated with HRP Streptavidin (BioLegend #405210), washed, and incubated with Colorimetric HRP substrate (BPS Bioscience #79651). The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.    

Description: Recombinant human CD20, also known as membrane spanning 4-domains A1 (MS4A1), full length, encompassing amino acids 1-297(end). This construct contains a C-terminal FLAG-tag. This recombinant protein was affinity purified.

Format: Aqueous buffer solution

Formulation: 50 mM HEPES (pH 8), 150 mM NaCl, 10% (vol/vol) glycerol, 0.1% DDM, 0.01% CHS, and 100 µg/ml FLAG peptide

Genbank: NM_021950.3

Host Cell Line: HEK293

Purity: ≥85%

Storage Stability: At least 6 months at -80°C

Tags: C-terminal FLAG-tag

Uniprot: P11836

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS101572-2
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 101572-2
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK
• Wirt: Human