CD20 Positive Cell Isolation Kit

Products from BPS Bioscience require a minimum order value above 400€

Application: Isolate or deplete CD20-expressing cells from a mixed population such as PBMCsPositively selected cells, or CD20 cells (depleted population), may be used for downstream applications such as genomic analysis, expression assays, protein isolation or flow cytometry

Background: B cells are critical immune cells for functions such as antibody production, support of other mononuclear and antigen-presenting cells, and they influence inflammatory pathways. CD20 is a marker of certain B cell subsets, which include B cell blasts, B cell malignancies, pre-B cells, naÍve, mature, and activated B cells. There is also a small subset of CD20⁺ T cells in PBMCs. In PBMCs derived from healthy individuals, about 6-23% of the cells are CD20⁺ cells.

Description: The CD20 Positive Cell Isolation Kit is designed to magnetically separate CD20-expressing cells from a complex immune cell population. This kit is optimized for the isolation of CD20 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, the CD20 positive cells are immobilized along the side of the tube while CD20-negative cells remain in suspension and can be easily removed.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Supplied As: This kit provides enough reagents and materials for isolation of CD20+ cells from up to 1 x 10⁸ or 1 x 10⁹ PBMCs. It is possible to use this kit for multiple isolations from smaller PBMC amounts.

Uniprot: P11836

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

• Artikelnummer: BPS82132-1
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 82132-1
• Verpackungseinheit: 1 x 10⁸ Cells
• Mengeneinheit: STK