CD226:CD112 Homogeneous Assay Kit

Products from BPS Bioscience require a minimum order value above 400€

Applications: Useful for screening for inhibitors of CD226 binding to CD112

Background: CD226 is an activating receptor expressed on the surface of natural killer (NK)cells, monocytes, platelets, and T cells. Its natural ligands are CD112 and CD155 which areexpressed by antigen presenting cells (APC). Binding of CD226 to either CD112 or CD155triggers NK cell effector function and can promote Th1 cell differentiation in T cells. CD226competes for binding with CD112 and CD155 with the co-inhibitory receptor TIGIT. AgonisticCD226 monoclonal antibodies are being explored as potential therapeutics in settings whereimmune activation is beneficial, such as cancer.

Contraindications: Only limited amounts of DMSO can be included, as it has been shown to disrupt CD226:CD112 interaction. Avoid green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.

Description: The CD226:CD112 Homogeneous Assay Kit is designed to measure theinhibition of CD226 binding to CD112 (PVRL2). The CD226:CD112 Homogeneous Assay Kitcomes in a convenient AlphaLISA® format with purified biotinylated CD226, His-tagged CD112,and assay buffer to perform a total of 384 reactions. With this kit, only three simple steps on amicrotiter plate are required. First, a sample containing CD226 and an inhibitor of choice isincubated with the CD112 for 60 minutes. Next, acceptor beads are added, then donor beads,followed by reading the Alpha-counts.

Storage Stability: Stable for at least one year from date of receipt when stored as directed.

Biosafety Level: Not applicable (BSL-1)

References: 1. Bottino, C. et al., J. Exp. Med. 2003; 198(4): 557-567.
2. Pende, D. et al., Blood. 2005; 105(5): 2066-2073.

• Artikelnummer: BPS72051
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 72051
• Green Labware: Nein
• Verpackungseinheit: 384 rxns.
• Mengeneinheit: PAK