CD27 antibody (O323) (PE)

Clone Name: O323

Light Chain: Kappa

Product Description: The O323 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of ≥ 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been included within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells. The O323 antibody may be used for analysis of CD27 expression on peripheral T cells, and is frequently used in combination with antibodies for IgD and IgM to distinguish naïve vs. memory B cell populations. For identification of Breg cells, this antibody has been used in combination with antibodies for CD25, CD1d, IL-10 and TGF-beta. The antibody is also reported for cross-reactivity with Baboon, Cynomolgus and Rhesus CD27.

Application Note: FACS: 0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Calculated MW: 29

Form: Liquid

Buffer (with preservative): 10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin, 0.09% Sodium azide.

Concentration: 0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Background: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]

Uniprot ID: P26842

Purification: Purified by affinity chromatography

Conjugation: PE

Full Name: CD27 molecule

• Artikelnummer: GTX01457-08-100
• Hersteller: GeneTex
• Hersteller Artikelnummer: GTX01457-08-100
• Green Labware: Nein
• Verpackungseinheit: 100 test
• Mengeneinheit: PAK
• Reaktivität: Human, Primate, Cynomolgus Monkey (Primate), Rhesus Monkey (Primate)
• Klonalität: Monoclonal
• Methode: Flow Cytometry
• Isotyp: IgG1
• Human Gene ID: 939
• Wirt: Mouse