CD4 antibody (SK3) (PE-Cy7)

Clone Name: SK3

Light Chain: Kappa

Product Description: The SK3 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells. The SK3 antibody is widely used as a phenotypic marker for human CD4 expression, and has been reported to be cross-reactive with Rhesus and Cynomolgus CD4. This antibody does not block binding of alternative clone RPA-T4, suggesting that they recognize different epitopes.

Application Note: FACS: 0.06 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Calculated MW: 51

Form: Liquid

Buffer (with preservative): 10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin, 0.09% Sodium azide.

Concentration: 0.012 mg/ml (Please refer to the vial label for the specific concentration.)

Background: This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Uniprot ID: P01730

Purification: Purified by affinity chromatography

Conjugation: PE-Cy7

Full Name: CD4 molecule

• Artikelnummer: GTX01461-10-100
• Hersteller: GeneTex
• Hersteller Artikelnummer: GTX01461-10-100
• Green Labware: Nein
• Verpackungseinheit: 100 test
• Mengeneinheit: PAK
• Reaktivität: Human, Cynomolgus Monkey (Primate), Rhesus Monkey (Primate)
• Klonalität: Monoclonal
• Methode: Flow Cytometry
• Isotyp: IgG1
• Human Gene ID: 920
• Wirt: Mouse