CD4+ T cells, Negatively Selected (Human)

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Description: Cryopreserved vial (10 x 10^6 cells) of CD4+ T cells that were negatively selected from freshly isolated primary human peripheral blood mononuclear cells (PBMCs). The PBMCs came from a healthy donor, and were isolated from whole blood or leukapheresis samples using a Ficoll gradient. Magnetic antibodies to monocytes, granulocytes, CD8+ T cells, gamma/delta T cells and other immune subsets present in PBMCs were then used to purify untouched CD4+ T cells via immunomagnetic separation. Before and after CD4+ T cell isolation, the cells were stained to evaluate purity and viability by flow cytometry. Cells were cryopreserved in CryoStor CS10 cryopreservation medium (Stemcell, #07930) at a controlled rate.Source
Normal human PBMC from Leukapheresis Sample

Storage Stability: Store cells at -135°C or colder. Thawed cells should be used immediately for downstream applications. Because these are primary cells, we do not recommend maintaining these cells in culture for long periods of time.

Biosafety Level: BSL-1

• Artikelnummer: BPS79752
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 79752
• Verpackungseinheit: 10 million cells
• Mengeneinheit: STK
• Wirt: Human